High-performance liquid chromatography is used to separate, identify and quantify the different components in a liquid mixture. Separation is achieved by the components having different affinity for the mobile (liquid) phase being pumped through the system, and the stationary phase present in the separation column. A detector, usually for UV absorption, at the end of the system detects the intensity at either a certain wavelength or over a range of different wavelengths (diode array). This can then, assuming complete separation has been achieved, be used to quantify the different components using standard samples of known concentration.

Two Waters LC-UV/DAD 2795/2996 (LCDAD) systems. This robust HPLC apparatus allows flow-rates from 50ul/min to 5ml/min for use with 2.1 mm ID columns and larger. The autosampler accepts standard 96/384 well plates with programmable temperature control from 4 to 40°C. A heated column compartment provides temperatures from 5 degrees above ambient to 65°C. A range of LC detectors, like a dual wavelength- or PDA UV detector is optionally available.

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